Coding

Part:BBa_K4461013:Design

Designed by: SHIH-HSUN, YANG   Group: iGEM22_NYCU-Taipei   (2022-10-10)


mCherry+tag fusion protein


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 694
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 694
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 694
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 694
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 694
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We synthesized a commercial tag with EcoRI and HindIII cutting sites.


Source

gene synthesis

References